Properties of the Syrian hamster phosphomannosyl receptor: an aggregate of low molecular weight proteins.

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis

The effects of specific antibody-complement-mediated cytotoxicity on transformed and untransformed Syrian hamster cells.

Antibodies were produced against 220,000-molecular-weight proteins of Syrian hamster embryo cells. The antiserum containing these antibodies is capable of immunologically staining the surfaces and a fibrillar network around untransformed Syrian hamster embryo cells. The staining is removed by adsorption of the antiserum with Syrian hamster embryo cells and by mild trypsin treatment. Several lin...

Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates.

A novel sensitive binding assay for quantitation of a low-molecular-weight phosphomannosyl receptor (41-46 K) was devised. The receptor was immobilized by immunochemical means in the wells of polystyrene multiwell plates. The lysosomal enzyme ligand, testicular beta-galactosidase, was added and receptor-bound beta-galactosidase was measured by conventional colorimetric analysis using p-nitrophe...

Detergent dissociation of bovine liver phosphomannosyl binding protein.

We have reported previously the isolation and partial characterization of a 215-kilodalton (Kd) phosphomannosyl binding protein from bovine liver membranes [3,9]. In the present studies evidence is presented that the binding protein is an aggregate. Four N-terminal amino acids were detected, and the complex could be dissociated into subunits. Bovine liver membranes were extracted with the deter...

Spontaneous differentiated squamous cell carcinoma of cheek pouch in a Syrian hamster

A 2-year-old male hamster was presented because of left facial swelling and salivation. Necropsy wasperformed and affected cheek pouch with tissue samples of regional and internal organs were submitted forhistopathological study. In histopathological examination it was found that the tumor consisted of cords andislands of tumoric epithelial cells which were diffused in the dermis. Vacoulation o...